Table 1.

Sequences of the Primers

Primers	Sequences
Forward	5′ AGCTCTCGAGAAAAGATGCCACCTGCCTCACTCC3′
Reverse	5′ CCGGAATTCAACCAGGTGTGTGTCAGTCC3′
5′AOXI	5′GACTGGTTCCAATTGACAAGC3′
3′AOXI	5′GCAAATGGCATTCTGACATCC3′

The sequences underlined with a single line, double lines denoted the cleavage sites of Xho I and EcoR I, respectively; the shadowed sequences denoted the cleavage sites of Kex2.