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. 2015 Mar 1;8:135. doi: 10.1186/s13071-015-0736-2

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© Vieira et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Long-term experiment showing bacteria population in Rhodnius prolixus midgut infected with Trypanosoma rangeli . CFU counts were made with R. prolixus 5^th instar nymphs 12 days after feeding (DAF) on blood without parasites. Previously, 4^th instar nymphs were fed on inactivated blood with or without 1 × 10⁶ epimastigotes/mL. Bars represent mean ± SEM of three independent experiments. Each point represents the CFU from three pools of three insects (n = 9). Means were compared using Student T-test or Mann–Whitney test; **p < 0.01 and *p < 0.05.