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. 2015 Mar 1;8:135. doi: 10.1186/s13071-015-0736-2

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© Vieira et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Bacterial composition identified by 16S ribosomal pyrosequencing in Rhodnius prolixus midgut infected with Trypanosoma rangeli . Long-term experiment showing bacterial composition at the family levels. Pyrosequencing 454 experiments of anterior midgut preparations from R. prolixus 5^th instar nymphs 7 days after feeding (DAF) on blood without parasites. Previously, 4^th instar nymphs were fed on inactivated blood with or without 1 × 10⁶ epimastigotes/mL of T. rangeli. Each bar graph presents the mean number of sequence reads assigned to a given bacterial family in four insect samples. Others: represent families with only one or two sequences (Pseudomonadaceae, Comamonadaceae, Rhodobacteraceae, Phyllobacteriaceae, Bradyrhizobiaceae, Staphylococcaceae, Bacillaceae, Nitrospiraceae, Flavobacteriaceae). Means were compared using t-test or Mann–Whitney test; *p < 0.05.