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. 2014 Nov 8;5(24):12891–12907. doi: 10.18632/oncotarget.2632

Figure 1. Incubation effect on resveratrol-induced signal transduction, gene expression and anti-proliferation.

Figure 1

Figure 1

(A) MDA-MB-231 cells were treated with 10 μM resveratrol for different time periods (0.5 to 4 h) prior to harvest. Cell extracts were separated by SDS-PAGE and blotted with anti-pERK1/2. Number of independent experiments (N) = 3. (B) MDA-MB-231 cells were treated with 10 μM resveratrol for different time periods (0.5 to 4 h) prior to removal of resveratrol and re-fed with fresh medium before harvest. Cell extracts were separated by SDS-PAGE and blotted with anti-pERK1/2 and anti-ERK2. N = 4. (C) MDA-MB-231 cells were treated with 10 μM resveratrol for different time periods (1 or 4 h) prior to removal of resveratrol and re-fed with fresh medium before harvest. Total RNA was extracted and qPCR was conducted as described. Number of independent experiments (N) = 3. (D) MDA-MB-231 cells were seeded in Petri dish and re-fed with DMEM containing 10% serum. 10 μM (228.24 × 10−6 mg/ml) of resveratrol was added to cell culture daily for 24, 4, 2, 1 and 0.5 h. After incubation, cells were re-fed with fresh medium. Cells were harvested at the 6th day and cell numbers were counted. N = 4 (*p < 0.05, compared to control with vehicle solvent)