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. 2014 Oct 24;5(24):12835–12848. doi: 10.18632/oncotarget.2547

Figure 7. Alteration of downstream regulated migration ability.

Figure 7

Whole lysates of KRN633 treated HB1.F3.CE cells were extracted using protein extraction solution. Quantified proteins were then separated by SDS-PAGE and transferred to a PVDF membrane. For immunoblotting, the membrane was incubated with primary antibodies including anti-phospho-Erk1/2 (1:1,000 dilution), anti-phospho-Akt (1:1,000 dilution), and anti-c-fos (1:2,000 dilution). Each protein was normalized against the GAPDH protein. (A) Expression of p-Erk1/2, p-Akt, and c-fos in KRN633 treated HB1.F3.CE cells. (B) The relative value of p-Erk1 protein. (C) The relative value of p-Erk2 protein. (D) The relative value of p-Akt protein. Each experiment was conducted in triplicate and presented as the mean ± SD. *; p < 0.05 vs. negative control (no treatment with CPT-11 or HB1.F3.CE cells). #; p < 0.05 vs. CPT-11 treated cells without HB1.F3.CE cells.