Figure 3. Candidate genes were confirmed on RNA-sequencing data and validated by qPCR.

(A and B) The candidate genes obtained by analysis of microarrays were further confirmed on two RNA-seq database including 57 AII and 71 AIII (CGGA and TCGA); (C) The top 3 survival associated genes, GYS1, TGFB1I1 and GABRA3, were validated by qPCR in 6 cell lines (2 commercialized cell lines and 4 primary culture cells). (D) The expression level of TGFB1I1 was further validated on additional 49 samples (36 AIIs and 13 AIIIs). *p<0.05, **p<0.01, ***p<0.001