Figure 5. TGFB1I1 regulated TGF-β1 induced EMT.

(A) Correlation analysis performed in 310 samples with mRNA microarrays and a cluster of 308 TGFB1I1 associated genes were obtained (R>0.6, p<10⁻³⁰); Pathway analysis was performed using the 308 TGFB1I1 associated genes. (B) The expression level of TGFB1I1 was positively correlated with the expression level of TGF-β1 in the three databases. (C and E) TGFB1I1 and EMT associated markers were significantly increased in TGF-β1 stimulated cells and TGFB1I1 shRNA resulted in the decreasing expression of N-cadherin, vimentin, slug, β-catenin and MMP-2. (D) A significantly decreased invasion was observed in the TGFB1I1 shRNA treated cells compared with TGF-β1 stimulated cells. *p<0.05