Figure 1. Apoptosis and necrosis/late-apoptosis induced by TMZ, FM or vemurafenib.

Cells were treated with the chemotherapeutic and 120h later the response was assayed. Presented data are from at least three independent experiments. *p<0.05, **p<0.005, ***p<0.0001. For all alkylating agent experiments, MGMT was depleted using the specific inhibitor O⁶BG (10 μM) by adding it to the cells 1h before TMZ or FM. Response of BRAF mutant (A) and wild-type (B) cells following vemurafenib addition. Response of BRAF mutant (C) and wild-type (D) cells following TMZ or FM addition. (E) Induced cell death, obtained by combining apoptosis and necrosis/late-apoptotic data from figures 1A, 1B, 1C and 1D, for BRAF mutant versus wild-type cells.