Figure 2. Apoptosis and necrosis/late-apoptosis induced by combination treatment with TMZ and vemurafenib or FM and vemurafenib.

Cells were treated with the chemotherapeutic and 120h later the response was assayed. Presented data are from at least three independent experiments. *p<0.05, **p<0.005, ***p<0.0001. MGMT was depleted with O⁶BG (10 μM) 1h before TMZ or FM. Response of BRAF mutant (A) and wild-type (B) cells following vemurafenib addition in combination with either TMZ or FM. Vemurafenib was added to the cells 1h after TMZ or FM. (C) Induced cell death, obtained by combining apoptosis and necrosis/late-apoptotic data from figures 2A and 2B, for BRAF mutant versus wild-type cells.