Figure 4. Apoptosis and necrosis/late-apoptosis induced in BRAF mutant and wild-type melanoma cell lines.
Cell death induced in BRAF mutant (A375) (A) and wild-type (SK-Mel537) (B) cells following vemurafenib (5 μM) addition in combination with either TMZ or FM. MGMT was depleted with O6BG (10 μM) 1h before TMZ or FM. Vemurafenib was added to the cells either 1h after TMZ and FM (t0) or 72h after TMZ and FM (t72) addition. Apoptosis and necrosis/late-apoptosis was determined 120 h after TMZ or FM addition. Presented values are the sum of the apoptotic and the necrotic/late-apoptotic results. Presented data are from at least three independent experiments. *p<0.05, **p<0.005, ***p<0.0001.