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. 2014 Oct 21;5(24):12607–12620. doi: 10.18632/oncotarget.2610

Figure 5. Characterization of BRAF mutant cells that have acquired resistance to vemurafenib.

Figure 5

(A) Response of BRAF mutant (A375) and vemurafenib resistant BRAF mutant cells (iA375R) to vemurafenib. Cells were treated with vemurafenib and 120h later the response was assayed. (B) Immunoblots of MGMT, MSH2, MSH6, PMS2 and MLH1 in A375 and iA375R cells. ERK2 served as loading control. Immunoblots of MGMT protein (C) and MGMT activity (D) in BRAF mutant (A375) and vemurafenib resistant BRAF mutant cells (iA375R). Cells were treated with vemurafenib (5 μM) and samples were harvested at indicated times. HSP90 served as loading control. (E and F) Response of A375 and iA375R to TMZ (E) and FM (F). Cells were treated with the indicated concentrations of the alkylating agents and 120h later the response was assayed. Presented data are from at least three independent experiments. *p<0.05, **p<0.005, ***p<0.0001.