Table 1. Significantly metabolic alterations identified in SaOs-2 cells after TAp73 over expression.

SaOs-TAp73β	Dox 8h vs CTRL	Dox 16h vs CTRL	Dox 16h vs Dox 8h
Biochemicals p ≤ 0.05	55 (54/1)	42 (34/8)	17 (7/10)
Biochemicals 0.05 ≤ p ≤ 0.10	30 (26/4)	28 (15/13)	17 (5/12)

Significantly altered metabolites in SaOs-Tet-on. Red indicates number of upregulated compounds, whereas green indicates downregulation. Following log transformation and imputation with minimum observed values for each compound, Welch's two-sample t-test was used to identify biochemicals that differed significantly between doxycycline-induced sample groups compared to control (no doxycycline), as well as identifying significant biochemical migration as induction progresses from eight to sixteen hours of doxycycline exposure. An estimate of the false discovery rate (q-value) is calculated to take into account the multiple comparisons that normally occur in metabolomic-based studies; as q-values were reasonable for p ≤ 0.05, no q-value cutoff was established.