Skip to main content
. 2014 Dec 26;5(24):12694–12703. doi: 10.18632/oncotarget.3031

Figure 2. Menadione enhances oxygen consumption and depletes intracellular ATP.

Figure 2

A) Menadione promotes dose-dependent decrease in intracellular ATP levels in melanoma cells. ATP levels determined by a luminescent cell-based assay; n=3. B) HPLC determination of total nucleotides from cells that are treated with 20μM of MEN or vehicle for 1.5 hours. C) Oxygen consumption rate (OCR) was measured on a Seahorse analyzer. Oligomycin (1μM), vehicle (ethanol) or menadione (10-40μM), 2,4-dinitrophenol (DNP, 60μM), and a combination of rotenone (1μM) and antimycin A (1μM) were applied to Mel526 cells as indicated. Each data point represents mean OCR ± s.e. from 5 replicates. D) Superoxide levels measurements by DHE fluorescence in the presence of menadione.