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. 2014 Dec 10;5(24):12528–12542. doi: 10.18632/oncotarget.2985

Figure 1. A unique mRNA gene signature distinguishes salivary gland adenoid cystic cancer (ACC) from matched normal salivary gland tissue.

Figure 1

A) Principle component analysis (PCA) for all salivary gland adenoid cystic cancer (ACC) and matched normal samples. Red and green spheres indicate fusion positive and negative tumors respectively. Blue and brown indicate their matched normal tissues respectively. B) Supervised hierarchical-ward clustering analysis using 1160 mRNA probe sets that were significantly expressed in ACC (two sides paired t-test, FDR=0.05; see Supplementary Table 1 for complete gene list). Star depicts tumor sample with negligible MYB expression and matched normal tissue. MYB-NFIB fusion positive samples (P) and fusion negative samples (N). C) Gene localization and molecular enrichment analysis using Fisher's t-test (***, paired t-test, two side p-value<0.0001; **, paired t-test, two side p-value<0.001; ns, paired t-test, two side p-value>=0.05). Gene localization was defined by Ingenuity IPA. D) MYB-related network in ACC. The inner layer: MYB-interaction genes defined by Ingenuity IPA. The outer layer, MYB regulating genes defined by published ChIP-Seq data [33]. E) Average intensities of MYB probe sets for three subtypes of salivary gland tumors and their matched normal samples (***, paired t-test, two side p-value<0.0001). F) MYB average intensity in ACC for each sample. Arrow depicts sample with low MYB. G) qRT-PCR validation of relative MYB expression levels in ACC tumors (***, two sample t-test, two side p-value<0.0001; error bars are S.E.M. of 12 samples).