Skip to main content
. 2014 Dec 10;5(24):12528–12542. doi: 10.18632/oncotarget.2985

Figure 6. Functional analysis of MYB fusion protein.

Figure 6

A) Illustration of MYB cDNA clones tested fragments tested and their expression efficiency in human immortalized salivary ductal cells (DC). MYB-NFIB protein showed increased steady-state protein and B) prolonged half-life in human immortalized myoepithelial cells (MC). C) Forced expression of MYB-NFIB enhanced the anchorage independent colony formation of human salivary DC cells. ***, t-test, two side p-value<0.0001; ns, p-value >=0.05. Error bars are S.E.M. of 3 replicates for each group. D) Decreased attachment and increased viability of suspension cells after ectopic MYB-NFIB expression. Error bars are S.E.M. of 3 replicates for each group. E) ACC tumor cell growth inhibition after shRNAi depletion of VCAN, and F) decreased xenograft tumor growth ability in NOD.SCID mice (two sample t-test, error bars are S.E.M. for 4 replicates).