Figure 2. Effects of chemotherapy on the expression of Aurora-A and apoptosis-related proteins.
(A) Western blotting detection of Aurora-A protein expression in three HCC cell lines (HepG2, Hep3B and SMMC-7721) and a normal human hepatocyte cell line (HH). (B) MTT analysis of IC50 values of ADR and CDDP in three HCC cell lines (HepG2, Hep3B and SMMC-7721). (C) 24h after HCC cells (HepG2, Hep3B and SMMC-7721) were treated with ADR (0.5 μg/ml), Western blotting detection of Aurora-A, p-Aurora-A, PTEN, Bcl-2, Bax and cleaved caspase-3 proteins. (C) 24h after HCC cells (HepG2, Hep3B and SMMC-7721) were treated with CDDP (1.0 μg/ml), Western blotting detection of Aurora-A, p-Aurora-A, PTEN, Bcl-2, Bax and cleaved caspase-3 proteins. GAPDH was used as an internal control. Data were presented as mean ± SD of at least three independent experiments. N.S, P>0.05; *P<0.05; **P<0.01.