Figure 5. Effects of Aurora-A upregulation on in vitro chemosensitivity of HCC cells.
(A) qRT-PCR and Western blotting detection of Aurora-A mRNA and protein expression in stably transfected HepG2/Aurora-A or HepG2/control cells, respectively. GAPDH was used as an internal control. (B) MTT analysis of IC50 values of ADR or CDDP in HepG2/Aurora-A or HepG2/control cells, respectively. (C) The colony formation of HepG2/Aurora-A or HepG2/control cells treated with various concentrations of ADR (0.0 or 0.5 μg/ml) or CDDP (0.0 or 1.0 μg/ml). (D) Flow cytometric analysis of apoptosis in HepG2/Aurora-A or HepG2/control cells combined with various concentrations of ADR (0.0, 1.0 and 2.0 μg/ml) or CDDP (0.0, 1.5 and 3.0 μg/ml). Data are expressed as the mean±SD of three individual experiments. N.S, P>0.05; *P<0.05; **P<0.01.