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. 2015 Mar 5;9:14. doi: 10.3389/fnana.2015.00014

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Copyright © 2015 DeFelipe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dendritic spines stained with the methylene blue method. (A,B) Low and high magnification photomicrographs, respectively, of a preparation by Cajal of the hippocampus stained with the methylene blue method (preparation housed at the Cajal Institute). These photomicrographs are from unpublished material from DeFelipe and Jones (1988). Scale bar (in B): (A) 120 μm; (B) 10 μm. (C) Drawing used by Cajal to show the existence of dendritic spines on pyramidal cells with the methylene blue method. Taken from Cajal (1899–1904).