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MS/MS spectra of the 271–284 tryptic peptides from the alpha subunit of membrane-associated methyl coenzyme M reductase (McrA) show that the 1-N-methyl histidine residue signals its presence with strongly enhanced b-ion intensities. (A) M. mazei MM1240 H*AALVSMGEMLPAR, and (B) M. acetivorans MA4546 H*AALVSMoxGEMoxLPAR.
