Skip to main content
. 2014 Nov 17;81(1):40–52. doi: 10.1111/tpj.12691

Figure 2.

Figure 2

HaRxL106 and MOS6 form a stable complex in vitro with a Kd in the low micro-molar range.(a) Elution volumes of His6-tagged HaRxL106, HaRxL106ΔC and ΔIBBMOS6 on a Superdex HR 200 30/10 size exclusion chromatography column determined by absorption at 280 nm. The upper two panels show elution profiles of the three proteins alone. The lower two panels show elution profiles of mixtures of ΔIBBMOS6 with either HaRxL106 or HaRxL106ΔC at a molar ratio of 1ΔIBBMOS6:2HaRxL106(ΔC).(b) SDS-PAGE of fractions of ΔIBBMOS6/HaRxL106 and the ΔIBBMOS6/HaRxL106ΔC control eluting from the column.(c) ITC binding isotherms and associated fits for the interactions between His6–ΔIBBMOS6 and His6–HaRxL106, His6–HaRxL106ΔC or His6–SAP11. Kd values are representative of two ITC experiments.