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. 2013 Dec 17;77(1):119–135. doi: 10.1111/tpj.12371

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© 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Reduced pre-rRNA processing in rh57 mutants. Comparison of the relative levels of the various regions of pre-rRNA in 9-day-old WT Col-0 and rh57 mutant plants. (a) Primer sets specific to various regions of 45S/35S pre-rRNA were used for synthesis of the first-stranded cDNA and determination of the levels of corresponding RNA sequences by qRT-PCR analysis. (b) Signal values for each PCR product were compared between rh57 and Col-0 plants. Black bars represent ETS and ITS sequences, and white bars represent 18S, 5.8S and 25S rRNAs. The values represent the means ± standard deviation (SD) for two independent isolations of RNAs.