FIG. 2.
Evaluation of IL-17A+ KdGag197-205-specific lung CD8 T cells in KO mice compared with wild-type BALB/c. H-2d background IL-4 KO (black), IL-13 KO (white), STAT6 KO (stripes), and wild-type BALB/c (gray) mice (n=4 per group) were immunized i.n./i.m. with AE FPV-HIV/AE VV-HIV. At 14 days booster vaccination, KdGag197-205-specific T cell responses were measured by (a, b) IL-17A ICS and (c) IL-17A ELISpot (unlike the spleen, the lung samples were not negatively enriched for CD8 T cells due to small sample size) as described in the Materials and Methods section. The graphs represent IL-17A-expressing HIV-specific CD8 T cells per 1×106 CD8+ T cells as a pooled value. In ICS data, unstimulated cells were used as background controls and these values (ranging 0.01–0.02%) were subtracted from each sample before plotting the graphs. The data are representative of 3 independent experiments.