Skip to main content
. 2014 Oct 7;4(10):e458. doi: 10.1038/tp.2014.98

Figure 2.

Figure 2

ClpB immunization in mice. ClpB-immunized mice (ClpB+Adj) were compared with mice receiving adjuvant (Adj), PBS or controls (Ctr). (a) Body weight changes during 32 days of the study. Food intake and feeding pattern were studied during the last 2 weeks in the BioDAQ cages. Mean daily food intake (b), meal size (c) and meal number (d) during last 10 days of the study. (e) Food intake during 24 h after injection of α-MSH (100 μg kg−1 body weight, i.p.) or PBS. (f) Plasma levels of ClpB-reactive IgG before and after adsorption with 10−6 M α-MSH. (g) Affinity of anti-ClpB IgG shown as the dissociation equilibrium constants (KD values). (h) Plasma levels of α-MSH-reactive total IgG. (i) Affinity (KD) of anti-α-MSH IgG. (j) cAMP assay in human embryonic kidney-293 cells overexpressing MC4R after stimulation by α-MSH alone or together with IgG (0.5 mg ml−1) pooled from ClpB-immunized or from Adj-injected mice. (k) The cAMP assay was performed with IgG depleted from anti-α-MSH IgG. (a) Two-way repeated measurement analysis of variance (ANOVA) before α-MSH injection (100 μg kg−1 body weight, i.p.), P<0.0001, Bonferroni post tests *a at least, P<0.05 ClpB group vs Ctr; *b at least, P<0.05 Adj group vs Ctr.; *c, P<0.05, Student's t-test ClpB group vs PBS; and *d, P<0.05, Student's t-test ClpB group vs Ctr. (b) ANOVA P=0.0002, Tukey's post tests ***P<0.001, **P<0.01, #P<0.05, Student's t-test. (c) ANOVA P=0.007, Tukey's post tests **P<0.01. (e) Student's t-test, *P<0.05. (f, g) ANOVA P<0.0001, Tukey's post tests ***P<0.001 ClpB +Adj vs other groups, paired t-test ##P<0.01, ###P<0.001. (h) ANOVA P=0.0002, Tukey's post tests ***P<0.001, *P<0.05; (i) Kruskal–Wallis test P=0.003, Dunn's post test **P<0.01, (mean±s.e.m., n=8). (j) ANOVA P=0.005, Tukey's post test *P<0.05; ANOVA P=0.04, Student's t-test #P<0.05, aClpB vs α-MSH, bClpB vs Adj. (mean±s.e.m.; j, n=6, k, n=3).