Table 1.

Proportion of positive cases per dilution, for each studied volume, Preah Vihear, Cambodia 2013^*

Parasite concentration		5 μL DBS		50 μL blood		200 μL blood		1 mL blood
p/μL	p/mL	No. of positive	%	No. of positive	%	No. of positive	%	No. of positive	%
1,000	1,000,000	21/21	100	21/21	100	21/21	100	21/21	100
100	100,000	21/21	100	21/21	100	21/21	100	21/21	100
10	10,000	21/21	100	21/21	100	21/21	100	21/21	100
1	1,000	11/21	52	21/21	100	21/21	100	21/21	100
0.1	100	1/21	5	19/21	90	20/21	95	20/21	95
0.01	10	N/A		0/21	0	7/21	33	2/21	10
0	0	0/21	0	0/21	0	0/21	0	0/21	0

^*Each dilution was tested in triplicate in seven polymerases chain reaction (PCR) assays (N = 21).

DBS = dried blood spots.