Table 2. Splice site calculations using HSF, BDGP and NetGene2 for splice site mutations found in the present study.

DNA variationa	Amino-acid exchange	HSFb		NetGene2c		BDGPd
		Wt	Mut	Wt	Mut	Wt	Mut
c.475-2A>G	p.Thr159_Leu209del	93.01	—	0.83	—	0.99	—
c.627+1G>C	p.Thr159_Leu209del	94.09	67.26	0.47	—	0.98	—
c.628-2A>G	p. Val210Aspfse21	95.05	66.11	0.92	—	0.95	—
c.954+2 T>A	p. Gly257Glufse2	87.31	—	0.91	—	0.98	—
c.1204-1G>A	p. Val402Phefse6	96.47	67.53e	0.77	—	0.99	—f

^aTranscript reference: NM_005857.4.

^bhttp://www.umd.be/HSF/.

^chttp://www.cbs.dtu.dk/services/NetGene2/.

^dhttp://www.fruitfly.org/seq_tools/splice.html.

^eNew splice site considered with 83.93.

^fNew splice site considered with 0.9.