Table 3. Validity of PCR-PGD analysis compared with embryo reanalysis (n=940 embryos, including aberrant).
| No. of subjects | Validity | ||||||
|---|---|---|---|---|---|---|---|
| TP | FN | TN | FP | Se (95% CI) | Sp (95% CI) | Accuracy (95% CI) | |
| Overall | 652 | 5 | 229 | 54 | 99.2% (98.2–99.8%) | 80.9% (75.8–85.3%) | 93.7% (91.9–95.2%) |
| By centre | |||||||
| E | 42 | 0 | 15 | 1 | 100% (91.6–100%) | 93.8% (69.8–99.8%) | 98.3% (90.8–99.9%) |
| C | 46 | 0 | 0 | 3 | 100% (92.3–100%) | – | 93.9% (83.1–98.7%) |
| A | 355 | 5 | 98 | 29 | 98.6% (96.8–99.5%) | 77.2% (68.9–84.1%)a | 93.0% (90.4–95.1%) |
| D | 21 | 0 | 22 | 8 | 100% (83.9–100%) | 73.3% (54.1–87.7%)a | 84.3% (71.4–93.0%)a |
| G | 154 | 0 | 76 | 5 | 100% (97.6–100%) | 93.8% (86.1–98.0%) | 97.9% (95.1–99.3%) |
| B | 34 | 0 | 18 | 8 | 100% (89.7–100%) | 69.2% (48.2–85.7%)a | 86.7% (75.4–94.0%)a |
| No. of cells used in PGD | |||||||
| One cell | 337 | 3 | 152 | 42 | 99.1% (97.4–99.8%) | 78.3% (71.9–83.9%) | 91.6% (88.8–93.8%)b |
| Two cells | 300 | 2 | 76 | 11 | 99.3% (97.6–99.9%) | 87.4% (78.5–93.5%) | 96.7% (94.3–98.2%)b |
| >2 cells (3 or 4) | 15 | 0 | 1 | 1 | 100% (78.2–100%) | 50% (1.2–98.7%) | 94.1% (71.3–99.9%) |
| Disease category | |||||||
| AR | 123 | 1 | 96 | 26 | 99.2% (95.6–99.9%) | 78.7% (70.4–85.6%) | 89.0% (84.4–92.6%)c |
| AD | 442 | 3 | 104 | 25 | 99.3% (98.0–99.9%) | 80.6% (72.7–87.0%) | 95.1% (93.0–96.7%)c |
| XL-R | 43 | 1 | 20 | 2 | 97.8% (88.0–99.9%) | 90.9% (70.8–98.9%) | 95.4% (87.3–99.0%) |
| XL-D | 44 | 0 | 9 | 1 | 100% (91.9–100%) | 90.0% (55.5–99.7%) | 98.1% (90.1–99.9%) |
| PCR-PGD protocol | |||||||
| Singleplex | 192 | 4 | 45 | 14 | 97.9% (94.8–99.4%)b | 76.3% (63.4–86.4%) | 92.9% (89.1–95.8%) |
| Multiplex | 460 | 1 | 184 | 40 | 99.8% (98.8–99.9%) | 82.1% (76.5–86.9%) | 94.0% (92.0–95.7%) |
| Combined biopsy–PGD-PCR strategies | |||||||
| Singleplex one cell | 67 | 3 | 21 | 8 | 95.7% (87.9–99.1%)c | 72.4% (52.8–87.3%) | 88.9% (81.0–94.3%)c |
| Singleplex two cells | 118 | 1 | 24 | 5 | 99.2% (95.4–99.9%) | 82.8% (64.2–94.2%) | 95.9% (91.4–98.5%) |
| Multiplex one cell | 270 | 0 | 131 | 34 | 100% (98.6–100%)c | 79.4% (72.4–85.3%) | 92.1% (89.2–94.5%) |
| Multiplex two cells | 182 | 1 | 52 | 6 | 99.5% (97.0–100%) | 89.6% (78.8–96.1%) | 97.1% (94.1–98.8%)c |
| Embryo morphology | |||||||
| Class 1 | 178 | 0 | 44 | 15 | 100% (97.9–100%)b | 74.6% (61.6–85.0%) | 93.7% (89.8–96.4%) |
| Class 4 | 37 | 2 | 21 | 1 | 94.9% (82.7–99.4%) | 95.5% (77.1–99.9%) | 95.0% (86.3–98.9%) |
a
P<0.05 for comparison with the centre G after Bonferroni correction.
b
P<0.05.
c
P<0.05 after Bonferroni correction.