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. 2015 Feb 19;2015:976575. doi: 10.1155/2015/976575

Figure 3.

Figure 3

let-7b expression in cells inhibited viral protein and gene expression of miRT-H1N1. Endogenous let-7b in HBE cells diminished viral PB1 gene (a) and M gene (b) expression with miRT-H1N1 infection. HBE or HEK293 cells were infected with miRT-H1N1, scbl-H1N1, or unmodified wt H1N1 at MOI 1 and, at 24 h p.i., cell lysates were examined by western blot analysis with antibodies against H1N1 PB1, M, or actin. The expression of PB1 or M is relative to that of actin. (c) IFA of H1N1 M in HBE cells infected with miRT-H1N1, scbl-H1N1, or wt H1N1 at 24 h p.i. after infection. Reduced expression of M from miRT-H1N1 was visualized by IFA in HBE cells using a H1N1 M-specific monoclonal antibody, and representative photomicrographs are shown at 400x magnification. HBE or HEK293 cells were transfected with anti-mir let-7b or ctrl anti-mir and the amount of viral PB1 (d) and M (e) was measured with quantitative PCR from infected HBE and HEK293 cells at 24 h after infection. Human GAPDH was an internal control. Data are mean ± SD of 3 independent experiments. * P < 0.05.