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. 2015 Feb 12;15:18. doi: 10.1186/s12935-014-0154-0

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© Liu et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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S100A7 knockdown inhibits proliferation in ANCI-H520 cells. S100A7 knockdown is stable over several cell passages with corresponding persistent decrease in growth rate of lung cancer cells. A. Quantitative reverse-transcription polymerase chain reaction confirms decreased messenger RNA (mRNA) over several cell passages 1 through 7. B. Analysis of proliferation using the same cell passages 1 through 7 shows a stable reduction in the growth rate of NCI-H520 knockdown cells. Controls for the experiments are wild-type cells and vector controls (NT). Data are presented as mean ± standard error of the mean; n =3 performed in triplicate. *P < 0.05.