Figure 1.

Mcl-1 depletion radiosensitizes pancreatic cancer cells. Panc-1 or BxPC-3 cells were treated with either non-specific (siNS) or Mcl-1 siRNA (siMcl-1). Seventy-two hours post-transfection cells were treated with radiation (0-8Gy). Radiosensitization was assessed by clonogenic survival and is indicated by an RER (radiation enhancement ratio) of greater than 1. Mcl-1 protein depletion by siRNA is shown (inset). Data are from a single representative experiment (A, B) or are the mean ± standard error of 3 independent experiments (C). Statistical significance versus siNS is indicated (*P<0.05). Cytotoxicity is the surviving fraction of siMcl-1-treated cells relative to siNS, in the absence of radiation.