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Mxi1-0, Mxi1, and Mxiex2-6 expression in SHEP and SHEP/MYCN cells. SHEP and SHEP/MYCN cells were transfected with pcDNA3.1 containing MXI1-0-HA, MXI1-HA, or MXIex2-6-HA. After 48 hours, the cells were fixed with methanol/acetone and incubated with a primary antibody to HA followed by a secondary antibody tagged with Alexa Fluor 488 (SHEP—green) or 594 (SHEP/MYCN—red). Cells were visualized with a CoolSnap CCD camera on a Nikon Eclipse E600 fluorescent microscope.
