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SHEP and SHEP/MYCN cells have different patterns of apoptosis in low serum in the presence of Mxi1 and Mxi1-0. SHEP/MYCN (A) and SHEP (B) cells were transfected with either pcDNA3.1 empty vector or pcDNA3.1 containing MXI1-0-HA, MXI1-HA, or MXIex2-6-HA. Cells were plated and cultured in RPMI-1640 containing low serum (0.5% FBS) for 48 hours. The cells were then fixed before incubation with a primary antibody to activated caspase-3 for immunohistochemistry.
