Skip to main content
. Author manuscript; available in PMC: 2015 Jun 17.
Published in final edited form as: Sci Transl Med. 2014 Dec 17;6(267):267ra176. doi: 10.1126/scitranslmed.3008411

Fig. 5. Enhanced TGFβ signaling in dystrophic muscles correlates with increased Wnt activity.

Fig. 5

(A) Representative Western blots of active β-catenin and TGFβ2 in diaphragms of WT and mdx5Cv mice at the indicated ages. Ponceau S stain was used as a loading control. (B) Diaphragms of 6-month-old mdx5Cv and WT mice were stained with antibodies recognizing TGFβ2 and eMyHC. (C) RT-PCR analysis of transcript amounts of the Wnt target genes LGR5 and TGFβ2 in YFP+ve cells purified from hindlimb muscles of 8-to 12-month-old Pax7CreER;R26RYFP;mdx5Cv mice (N = 4). Data are expressed as fold increase in expression compared to that for age-matched Pax7CreER;R26RYFP;WT mice (N = 4). (D) Diaphragms of 6-month-old mdx5Cv mice were stained for TGFβ2 and Axin2, and the average intensity of each signal was evaluated in 120 randomly selected regions of the muscles (see Materials and Methods). The correlation between the values obtained for each staining in each region is shown. Linear regression is indicated. Pearson r, 0.71 (P < 0.01; two-tailed t test).