Fig. 3.

Detection of latent HIV or dengue virus at low levels in macrophages and astrocytes, respectively. Merged images of HIV (a) or dengue-1 (b) staining (FITC, green staining) and actin staining (phalloidin conjugated to Texas red, red staining) demonstrate low to undetectable levels of infection. Numbers of positive infected cells were calculated using nuclear staining, DAPI. The merge of these three colors (Merge of the two cytoplasmic markers, HIV-p24 and actin, yellow plus nuclear staining) is also illustrated. In the case of HIV, HIV-p24 production was not detected by ELISA. In dengue infection, only 3% of human astrocytes were infected. These results were obtained using spinning disk and A1 confocal microscopy, respectively. Bar: 50 µm