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. Author manuscript; available in PMC: 2015 Mar 5.
Published in final edited form as: Nat Commun. 2014 Apr 7;5:3592. doi: 10.1038/ncomms4592

Figure 1.

Figure 1

Imbalance of amino acids and high levels of Met decrease lifespan and reproduction. a) Survivorship curve, mean lifespan, and lifetime eggs production of mated female Canton-S flies reared on the 0.1X or 1.0X amino acids diets containing 0, 1, 1.5, 10, or 100 mM Met were analyzed. Bar graphs show lifetime egg production (left axis) (mean±s.d., n = 40, *: p<0.05, **: p<0.01, Student’s t-test) and line graphs mean lifespan (right axis) (mean±s.e.m., n = 144 ~ 205, ***: p<0.0001, Log-rank test). See Supplementary Tables 1 and 2 for n number and statistics of individual dietary groups. b) Cumulative eggs production of mated female Canton-S flies reared on the 0.1X diet containing 0, 0.15, 1, or 10 mM Met for 30 days (mean±s.d., n =40). c) Cumulative eggs production of mated female Canton-S flies reared on the 1.0X diet containing 0, 1.5, 10, or 100 mM Met for 30 days (mean±s.d., n = 30).