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. Author manuscript; available in PMC: 2015 Mar 5.
Published in final edited form as: Nat Commun. 2014 Apr 7;5:3592. doi: 10.1038/ncomms4592

Figure 7.

Figure 7

Lifespan extension of virgin female flies and yeast by Met restriction. a–c) Survivorship curves of virgin Canton-S flies reared on a) 0.4X diet containing 0, 0.15, or 1 mM Met, b) 0.7X diet containing 0, 0.15, or 1 mM Met, or c) 1.0X diet containing 0, 0.15, or 1 mM Met, are shown. d) Mean lifespan of virgin flies on these diets was compared (mean±s.e.m., n = 92 ~ 111, ***: p<0.0001, Log-rank test). See Supplementary Tables 8 and 9 for n number and statistics of individual dietary groups. e) Survivorship curves and f) mean lifespan of yeast grown on defined medium containing 1, 0.1, 0.5, 0.05, or 0.005 mg/ml Met (mean±s.e.m., n = 30, **: p<0.01, Rank-sum test). g) Survivorship curve and h) mean lifespan of yeast grown on defined media containing 0.5 or 0.05 mg/ml Met under conditions of 0.2X or 1.0X amino acid status (mean±s.e.m., n = 30, **: p<0.01, Rank-sum test ). See Supplementary Tables 10 and 11 for statistics of individual dietary groups.