Figure 2.
Design of recombinant vectors. (a) Schematic design of scaffold proteins containing fluorescent protein mCherry, one, four and eight SRC Homology 3 (SH3) domains and a single guanosine triphosphatase (GTPase)-binding domain (GBD) under the control of xylose-inducible promoter; (b) Schematic design of recombinant cell fate proteins PleC, DivJ and DivK. They are fused with GBD peptide or SH3 peptide to interact with scaffold proteins. Cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) are used for fluorescent microscopy.