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. Author manuscript; available in PMC: 2015 Mar 5.
Published in final edited form as: J Biomed Sci Eng. 2013 Feb;6(2):134–143. doi: 10.4236/jbise.2013.62017

Figure 4.

Figure 4

Perturbation of spatial localization of cell fate determinants by scaffold proteins. (a) Strains carrying DivK-SH3pep-YFP, PleC-GBDpep-CFP or DivJ-GBDpep-CFP still localize properly when each is co-expressed with individual scaffolds (mCherry-SH3(1)-GBD, mCherry-SH3(4)-GBD and mCherry-SH3(8)-GBD); (b) Co-localization of PleC with DivK at both poles in the strain PB021 which expresses PleC-GBDpep-CFP, DivK-SH3pep-YFP and mcherry-SH3(8)-GBD; (c) Scanning electron microscopy (SEM) revealed that the localization of PleC to both poles did not lead to morphological change in dividing C. crescentus as compared to control strain PB018 which expresses only PleC-GBDpep-CFP and DivK-SH3pep-YFP; (d) Co-localization of DivJ with DivK at both poles in strain PB025 which expresses DivJ-GBDpep-CFP, DivK-SH3pep-YFP and mcherry-SH3(8)-GBD; (e) SEM revealed that the localization of DivJ to both poles did not lead to morphological change in dividing C. crescentus as compared to control strain PB022 which expresses only DivJ-GBDpep-CFP and DivK-SH3pep-YFP; (f) Growth rate of different genetically modified strains. The growth curve showed that the perturbation of cell fate proteins did not significantly alter growth rate. PB018: PleC-GBDpep-CFP, DivK-SH3pep-YFP; PB021: PleC-GBDpep-CFP, DivK-SH3pep-YFP, mCherry-SH3(8)-GBD; PB022: DivJ-GBDpep-CFP, DivK-SH3pep-YFP; PB025: DivJ-GBDpep-CFP, DivK-SH3pep-YFP, mCherry-SH3(8)-GBD. N = 3, scale bars represent SEM.