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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cancer Res. 2014 Dec 12;75(1):203–215. doi: 10.1158/0008-5472.CAN-13-3563

Figure 5. Chemotactic movement of breast tumor cells is affected by reducing acetylated α-tubulin.

Figure 5

(A–C) Real-time migration was analyzed with the xCELLigence system. Modified electronic chamber plates (CIM-plates) were utilized. Each cell line was plated in triplicate into serum-free media (upper chamber) and allowed to migrate towards the lower chamber (containing 5% FBS) for 24h. The serum-free control represents control cells plated in duplicate into serum-free media (upper chamber) where the lower chamber also contained serum-free media. Error bars indicate +/− standard deviation of the 3 experimental or 2 control wells/run. Graphs shown are representative of n=3 independent runs/cell line. Cell Index represents the change in electrical impedance representing movement from the upper to lower chamber. (A and B) Representative chemotaxis graphs of MDA-MB-231 or BT-549 stable pooled clones expressing the K40R α-tubulin mutant or the α-tubulin control and (C) MCF-7 cells transiently transfected with αTAT1-GFP or GFP control. The underside of the upper chamber was stained at 24h and representative images are shown to the right of each graph.