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. 2015 Mar 5;10(3):e0116374. doi: 10.1371/journal.pone.0116374

Fig 3. Generation of 1.3E2 clones expressing different amounts of NEMO protein.

Fig 3

(A) Work flowchart to generate NEMO reconstituted 1.3E2 cell clones. (B) An example of Western blots with different NEMO stable clones. Protein extracts from different 1.3E2 clones were separated on SDS-PAGE gel and immunoblotted with NEMO antibody. Tubulin was used as a loading control. (C) An example of immunofluorescence analysis with NEMO antibody (Green) in a NEMO reconstituted 1.3E2 stable clone. Nuclei were visualized by Hoechst staining (Blue).