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. 2015 Mar 5;10(3):e0116374. doi: 10.1371/journal.pone.0116374

Fig 4. Effects of NEMO amount on NF-κB activation.

Fig 4

(A) Cell extracts from equal numbers of 1.3E2 clones expressing different amounts of NEMO protein were generated using either 2x SDS Laemmli sample buffer or Totex buffer. The cell extracts were separated on SDS-PAGE gel and immunoblotted with NEMO, IKKα/β, and actin antibodies. (B, C) The 1.3E2 clones as in A were left untreated or treated with etoposide (VP16, 5 μM, 1 hr) in B or LPS (10 μg/ml, 30 min) in C. P represents pool of the 1.3E2 clones before isolation of individual clones. Protein extracts were examined by EMSA and Western blotting using NEMO, IKKα/β, and tubulin antibodies.