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. 2015 Mar 6;46:26. doi: 10.1186/s13567-015-0154-7

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© Wessel et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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RT-qPCR and PRV ơ1 staining from the 2 ^nd passage. (A) Mean PRV Ct value (and SD) detected by RT-qPCR in red blood cells (RBC) at 14 and 21 dpi (n = 6) during the second passage. Results are shown for cultures infected with undiluted and diluted (1:10) inoculum from the 1st passage. (B) Immunofluorescence microscopy picture showing the PRV ơ1-protein (green) in cells at 14 dpi infected by undiluted inoculum. Cell nuclei stained with Hoechst (blue).