Fig 5. Inhibition of Gβγ signaling suppresses intrinsically increased direct coupling of p-ERK1/2 and PDE4D proteins in asthmatic HASM cells.

Western blotting in Co-IP analysis demonstrating that, under the same protein loading conditions yielding similar β-actin levels, relative to normal cells, asthmatic HASM cells exhibit distinctly increased direct co-localization of immunoblotted (IB) PDE4D protein (apparent mol. wt. 100 kDa) with immunoprecipitated (IP) ERK1/2, the latter associated with heightened IB-detected p-ERK1/2. This heightened protein complex formation is acutely reversed (within 30 min) in asthmatic HASM cells treated with either anti-Gβγ blocking peptide or gallein. The immunoblots shown are representative of 3 experiments.