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. Author manuscript; available in PMC: 2015 Nov 26.
Published in final edited form as: Virus Res. 2014 Jul 24;193:89–107. doi: 10.1016/j.virusres.2014.07.001

Fig. 5. Double immunogold labeling shows enrichment of ABCE1 at sites of WT Gag assembly at the PM of HIV-1 infected cells.

Fig. 5

HIV-1-infected COS-1 cells expressing WT Gag or an assembly-incompetent MACA mutant were analyzed by immunogold double-label EM, using antibodies to Gag (small gold) and ABCE1 (large gold). Images were chosen to reflect previous quantitative immunolabeling studies that show significant recruitment of ABCE1 to WT Gag assembly sites at the PM, versus low levels of background ABCE1-Gag colocalization with the MACA mutant (Reed et al., 2012). (A) Six late WT assembly sites, half of which colocalize with ABCE1. (B) Three early WT assembly sites, all of which colocalize with ABCE1. (C) PMs of two adjacent cells expressing assembly-incompetent, membrane targeted MACA, displaying background levels of colocalization with ABCE1. Scale bars, 200 nm.