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. 2015 Mar 5;10(3):e0119282. doi: 10.1371/journal.pone.0119282

Fig 2. Fluorescence micrographs showing IFA-2::GFP variant localization in live adult transgene carrying lines.

Fig 2

Scale bars for FO and ALM micrographs are 10μm, for uterus micrographs the scale bars are 20 μm. A) Full length IFA-2::GFP fusion protein localizes to epidermal FOs in rescued ifa-2::gfp; ifa-2(nc16) animals. The GFP-dependent fluorescence is detected in thin stripes oriented perpendicular to the long axis of the worm in regions of the epidermis adjacent to muscle. B) Full length IFA-2::GFP fusion protein localizes to uterine seam and to C) ALM in ifa-2::gfp; ifa-2(nc16). D, E, F) Localization of headless IFA-2∆H::GFP protein is indistinguishable from IFA-2::GFP. Genotype shown is ifa-2 ∆H::gfp; ifa-2(nc16) G,H, I) Localization of IFA-2∆T::GFP protein is indistinguishable from IFA-2::GFP. Genotype shown is ifa-2 ∆T::gfp; ifa-2(+). J,K,L) Localization of IFA-2R::GFP protein is indistinguishable from IFA-2::GFP. Genotype shown is ifa-2 R::gfp; ifa-2(+).