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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Neurobiol Dis. 2014 Dec 29;75:15–30. doi: 10.1016/j.nbd.2014.12.016

Figure 9.

Figure 9

NP1 induction in brain following HI showed co-localization with pro-apoptotic activated caspase-3. A) Immunofluorescence analysis of activated caspase-3 in brain sections from WT and NP1-KO brains revealed the appearance of intense cleaved caspase-3 (i.e. activated) specific immunoreactive proteins in the WT ipsilateral cortex following HI. Whereas, caspase-3 specific immunofluorescence was almost absent in NP1-KO brains. B) Orthogonal sectioning shows co-localization of NP1 (red) and cleaved caspase-3 (green) in WT brain sections (seen as yellow). Scale bar, 5μm. C) Western blot analysis of cortical tissues from WT and NP1-KO brains collected at different times (6–48 h) post-HI showed cleaved caspase-3 immunoreactive protein bands (17 kDa) in the WT cortical tissue lysates but not in the NP1-KO cortical lysates. Representative bands are shown.