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. Author manuscript; available in PMC: 2016 Mar 3.
Published in final edited form as: Structure. 2015 Feb 12;23(3):472–482. doi: 10.1016/j.str.2015.01.003

Figure 2. Residues that mediate CHIP-TPR/Hsc70-lid interactions modulate CHIP-mediated ubiquitination.

Figure 2

(A) Western blot with anti-GST antibody to detect ubiquitination of GST-Hsc70395-646 by wild type (WT) and mutant CHIP1-303.

(B) Western blot with anti-CHIP antibody to detect autoubiquitination of wild type (WT) and mutant CHIP1-303.

(C) Pull-down of GST~Hsc70395-646 (GST~Hsc70-SBDβ-lid-tail) by wild type (WT) and mutant His6-CHIP1-303 bound to Ni2+ mag-sepharose beads analyzed by SDS PAGE with Coomassie staining. Lanes indicate GST~Hsc70395-646 and CHIP1-303 components utilized (INPUT), the final wash fraction (Wash) and the elution fraction (Elute). See also Figure S4.