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. Author manuscript; available in PMC: 2016 Mar 3.
Published in final edited form as: Cell Rep. 2015 Feb 26;10(8):1269–1279. doi: 10.1016/j.celrep.2015.01.056

FIGURE 4. MCU acutely controls muscle size in the adult.

FIGURE 4

A EDL muscles of adult mice (2-3 month old) were infected with AAV-MCU or AAV-shMCU. AAV-LacZ or AAV-shluc were used as negative controls, respectively. Two weeks later muscles were isolated and processed for further analysis. B-C Mean fiber size of AAV-MCU and AAV-shMCU infected muscles. >300 fibers per muscle; n=3. D Protein synthesis analysis. EDL muscles were infected with AAV-MCU for two weeks. Puromycin was then i.p. injected and muscles were isolated 30 minutes later. Left: western blotting with anti-puromycin antibodies. Ponceau S staining was used as loading control. Right: quantification. n=4. E-F Real-time RT-PCR analyses of AAV-MCU and AAV-shMCU infected muscles. n=4. G-H Left: immunoblotting of AAV-MCU and AAV-shMCU infected muscles. Right: quantification. n=4. I Expression pattern clustering according to Self-Organising Tree Algorithm (SOTA). Gene expression values are relative to the average expression in control condition (7 days). In panels B-H, data are represented as mean ± SEM. *p<0.05, **p<0.01, t test (two-tailed, paired).

See also Figures S4 and Tables S1, S2, S3.