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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Apoptosis. 2015 Apr;20(4):551–561. doi: 10.1007/s10495-015-1105-5

FIG. 6. Effect of HN and HN-L12A on Cyclophosphamide-induced male germ cell apoptosis.

FIG. 6

Mice were treated with vehicle (Control), HN, HN-L12A, CP, CP+HN, CP+HN-L12A, or CP+HN+HN-L12A as described in experimental procedures (n=5 each group). Apoptotic cell numbers were determined by TUNEL staining method. HN or HN-L12A alone did not change apoptosis compared with control. Compared with control, CP increased germ cell apoptosis both in early+late (I–IV and XI–XII) and middle (VII–VIII) stages. CP+HN significantly suppressed CP-induced apoptosis in early + late stages but not in middle stages. CP+HN-L12A showed no preventive effect against CP-induced apoptosis in neither early+late or middle stages. CP+HN’s preventive effect was completely blocked by CP+HN+HN-L12A. Values are means ± SEM. Means with unlike superscripts are significantly (P<0.05) different.