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. 2015 Mar 5;10(3):e0119372. doi: 10.1371/journal.pone.0119372

Fig 3. Examples of input and output pages of CRISPR MultiTargeter.

Fig 3

A. The input form for the multiple genes or transcripts input consists of the parameters for the sgRNA target site specification and the identifiers input area. B. The output page consists of the overall header indicating the type of design performed followed by the list of input identifiers which the user provided with the links to Ensembl gene pages where available. This example is from the multiple genes/transcripts workflow performed on sox9a and sox9b zebrafish genes. The main part of the output is focused on common sgRNA target sites and is organized in Visual and Table Views. The user can see the details of these views by clicking on the “expand or hide” links. Visual View consists of links to alignment with the target sites highlighted and markers for the start sites of target sites. Table View contains HTML tables with the relevant information on sgRNA target sites such as their ID numbers, sequences, start, end as well as computed sequence features such as GC % and predicted annealing temperature (Tm) of sgRNA:DNA interaction, exon numbers and predicted scores. There is also a “Unique sgRNA targets” part of the page which is organized similarly.