Fig 6. Effects of NiCl2 on LPS-induced changes in regnase-1 and Arid5a expression levels.
RAW264 cells were incubated in medium containing LPS (1 μg/ml) in the presence or absence of NiCl2 (300 μM) for the indicated periods (A and B) and 4 h (C). (A and B) Regnase-1, actin, and Arid5a protein levels in cell lysates were determined by immunoblotting. Protein levels of Arid5a and actin were quantified using ImageJ software (B). Data represent the mean ± S.E.M. (n = 4). Data are representative of three independent experiments. (D) Arid5a mRNA levels were measured by quantitative real-time PCR. Values are normalized to those of GAPDH and the mean value of the control was set to 1.0. Data represent the mean ± S.E.M. (n = 4). **p < 0.01 between the indicated groups. Data are representative of two independent experiments.